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Equipment

> Departments > Department of Soil and Plant System > Laboratory of Molecular and Environmental Microbiology > Equipment

Equipment

Genetic Analyzer 3130

Specifications:

4-capillary analyzer for electrophoresis separation of DNA fragments using capillary metod for sequencing and fragment analysis samples
Basecalling accuracy for sequencing – 98,5%
Basecalling accuracy for fragment analysis – 99,99%
Software for:
data collection and reporting,
microbial identification of bacteria and fungi based on sequence of rDNA,
Validated libraries of sequences of known microorganisms for identification of unknown microorganisms

Thermal cycler for Real-Time PCR 7500 Fast

Specifications:

Thermal cycling system based on Peltire system for 96-well plates or strip-tube – volume 0,1 ml
System with camera for fluorescence image registration
Detection in 5 chanels: -FAM/SYBR Green I, -VIC/JOE, – NED/TAMRA/Cy3, – ROX/Texas Red, – Cy5
Passive reference dyes: ROX Or Any calibrated dye

Gradient thermal cycler 9901 Veriti 96 well Fast Thermal Cycler

Specifications:

Thermal cycler with 96-well block (format 96 x 0,1 ml) made with 6 different areas controlled Rusing Peltier effect
Homogeneity temperature in the block +/-0,5°C
Posibility of regulation temperature between areas, as 4°C, and posibilty of setting the same temperature in all areas of the block
Temperature range from 4,0°C to 99,9°C with posibility setting accuracy for 0,1°C

The DCode Universal Mutation Detection System for DGGE analysis

Specifications:

System for DNA mutation analysis using DGGE technique (denaturing gradient gel electrophoresis)
System consists of temperature control module, two electrophoresis tank, one with ceramic cooling fingers and core
The system allows of two gels electrophoresis
The control module contains the stirrer for buffer mixing ans heater for temperature control 45-70°C
Power with voltage

Sub-Cell GT Agarose Gel Electrophoresis System

Specifications:

Aparatus for nucleic acids electrophoresis in agarose gel
Systems for separation of DNA restriction digestiong and polymerase chain re action-amplified fragments
Systems with UV-transparent plastic trays
Power with voltage power supply

Aparatus for DNA extraction

Specifications:

Work time from 1 to 60 second with increase as 1 s
Speed 4.0–6.5 m/s, with increase as 0.5 m/s
Adapter with 24 positions for 2 ml tubes
Cryogenic adapter holds 24 2-ml tubes

Spectrophotometer for DNA concentration analysis NanoDrop2000

Specifications:

Spectrophotometer UV/VIS for measurement samples in micro-volume
Sample volume – 0,5 μl
Range of wavelength – 190-840 nm
Measurement without cuvette, micro-cuvette and tips
Range of DNA concentration measurement: 2-15 000 ng/μl
Time of measurement cycle ≤ 5 sek

Gel documentation system

Specifications

System for vizualization, saling and analysis of fluorescent and colorimetric labeled samples
System with mini-darkroom with UV transilluminator and white light epi-illumination
Transilluminator equipped with UV lamps (302 nm UV-B)
System with filters for using ethidium bromie and Sybr-Green
Software can be used to image analysis, automatic identification of bands and dendrograms preparation

Automatic system for Fast microorganisms identification : Turbidimeter, Microstation, OmniLog, Microscope, Anoxomat, AeroSpray

Specifications:

System for automatic identification of bacteria: gram (-), gram (+), Bacillus, anaerobic bacteria, yeast and fungi
System for microbial identification on 96-well microplates with database containing 2000 of bacteria, yeast and fungi species
System with microstation reader for 96-well plates for identification and characterisation of microorganisms (anaerobic bacteria, yeast, fungi)
System with automatic station of bacteria identification (gram (+), gram (-) ), for incubation, reading and results analysis for 50 microplates 96-well (temperature incubation range from 22 to 45⁰C)
System equipped with turbidimeter, aparatus for Gram method staining, apparatus for microaerofiles and anaerobes incubation, optical microscope

Microplates counter INFINITE M200PRO

Specifications:

Aparatus with function of fluorimeter, photometric system and
Detection methods: absorbance with light source – UV xenon flashlamp, fluorescencje
Absorbance from 230 nm to 1000 nm
Reader for plates from 6 to 384-well plates
Read time of 96 –well plate – 20 sec
Reader with quarto plate for 16 measurement of DNA, RNA and protein concentration in 2 ml
Aparatus with shaker

The autopreparator and sterylizator of microbiological media Masterclave 09

Specifications:

The machine for media and diluents preparation in the range from 1 to 9 l
The machine equipped with magnetic stirrer – continuous work 40 rpm
Sterilisation temperaturę in the range 95-125⁰C
Sterilisation time in the range 1-180 min.
Dispensing temperature 25-80⁰C
Constant temperaturę during suplement addition

Automatic diluter Dilumat S

Specifications:

Dispensing speed – to 200 ml/min
Automatic distribution nozzle
Range of dilution: from 1:2 to 1:99
Weight of the sample 0 – 2500 g

Automatic Petri dish filling system APS One

Specifications:

Flow rate: to 650 dishes during 1 hour
Dispensing volume in the range from 1 to 30 ml
Manual dispensing from 1 to 1000 ml
Equipped with cooling system
Dispensing in sterile UV-chamber
Opening time of the plate < 3 sec.
Medium mixing during dispensing

Colony counter Easy Count2 7510/AES

Specifications:

Automatic counting of colony from Petridish (90mm) from pour, deep and spiral plate colony
Posibility of colony counting from different type of agar
Possibility of areas create from which colony will be counted
Automatic colony classification depending on the color
Automatic separation of overlapping colony
Possibility of results archiev

Pipetting stadion for PCR

Specifications:

Automated pipetting system to dispense quantities of liquid in the volume 1 µl to 1000 µl
System for 24, 48, 96 i 384-well plates and 0,2; 0,5; 1,5 oraz 2,0 ml tubes

Autoclave FVG2

Specifications:

Capacity 75 l
Range of temperature: 105-138^oC
Autoclave chamber made of stainless steel

Automated screening AS200 Control

Specifications:

Maximum capacity – 3 kg
Amplitude range from 0,2 to 3,0 mm
Dry and wet sieving

Sammler for microbiologiacl analysis of the air Sampl’air

Specifications:

Capacity 100 l for minute
For standard Petridish 90 mm
Possibility of delayed working: 00:00 do 00:59

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