{"id":16352,"date":"2017-03-18T12:06:32","date_gmt":"2017-03-18T11:06:32","guid":{"rendered":"\/en\/departments\/department-of-soil-and-plant-system\/laboratory-of-molecular-and-environmental-microbiology\/oferta-uslug\/"},"modified":"2017-05-08T12:12:27","modified_gmt":"2017-05-08T10:12:27","slug":"offer","status":"publish","type":"page","link":"https:\/\/www.ipan.lublin.pl\/en\/departments\/department-of-soil-and-plant-system\/laboratory-of-molecular-and-environmental-microbiology\/offer\/","title":{"rendered":"Offer"},"content":{"rendered":"<div class=\"wpb-content-wrapper\" id=\"wpb-content-root\"><p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text]<strong><em><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-14723\" src=\"\/wp-content\/uploads\/2017\/03\/dc2901e83873df456477acfd05a6578279929ea4-300x280.jpg\" alt=\"\" width=\"300\" height=\"280\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/dc2901e83873df456477acfd05a6578279929ea4-300x280.jpg 300w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/dc2901e83873df456477acfd05a6578279929ea4.jpg 658w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/em><\/strong><\/p>\n<p><strong><em>Preparation and storage of samples<\/em><\/strong><\/p>\n<ul>\n<li>Preparation and dispensing of microbiological culture media<\/li>\n<li>Sterilization of microbiological media, small glass and laboratory equipment<\/li>\n<li>Environmental samples homogenization<\/li>\n<li>Preparation of inoculating fluids and diluter used in microbiological analyses<\/li>\n<li>Preparation of microscope slides and automatically stain using Gram method<\/li>\n<li>Preparation and culturing of fungi in microcultures<\/li>\n<li>Storage of bacterial and fungal strains in microbanks in low temperature freezer (to -85 \u00b0 C)<\/li>\n<\/ul>\n<p><strong><em>Analyses based on molecular biology techniques<\/em><\/strong><\/p>\n<ul>\n<li>Selection and optimization of the DNA isolation methods from environmental samples, mycelium and bacteria and yeast cells<\/li>\n<li>Identification of bacteria based on 16S rRNA sequencing analysis<\/li>\n<li>Identification of fungi based on D2 fragment sequencing, located within the gene encoding the large subunit of the ribosome (LSU)<\/li>\n<li>Analysis of genetic profile of microorganisms by amplified fragment length polymorphism (AFLP)<\/li>\n<li>Phylogenetic analysis of bacterial and fungal strains isolated from environmental samples<\/li>\n<li>Genetic diversity of bacterial and fungal communities using polymerase chain reaction and denaturing \u00a0gradient gel electrophoresis (PCR-DGGE) and terminal restriction fragment length polymorphism (t-RFLP)<\/li>\n<li>Detection of microorganisms,\u00a0<span id=\"result_box\" class=\"short_text\" lang=\"en\">especially<\/span> heat resistant fungi by the moloecular biology methods (PCR, qPCR, LAMP)<\/li>\n<li>Detection and<span id=\"result_box\" class=\"short_text\" lang=\"en\"> expression analysis of genes encoding cellulose degradation enzymes (PCR, qPCR, qRT-PCR)<\/span><\/li>\n<\/ul>\n<p><strong><em>Analyses based on BIOLOG System<\/em><\/strong><\/p>\n<ul>\n<li>Identification and characterization of metabolic profile of fungi (FF plates)<\/li>\n<li>Identification and characterization of metabolic profile of bacteria(GEN III plates)<\/li>\n<li>Identification and characterization of metabolic profile of yeast (YT plates)<\/li>\n<li>Identification and characterization of metabolic profile of anaerobic microorganisms(AN plates)<\/li>\n<li>Functional microbial diversity evaluation based on characterization of soil metabolic profile (ECO plates)<\/li>\n<li>Microorganisms analysis by phenotype microarrays method (PM plates)<\/li>\n<li><span id=\"result_box\" lang=\"en\">Evaluation of microorganisms sensitivity to chemical compounds, plant extracts and fungicides (MT2 plates)<br \/>\n<\/span><\/li>\n<li><span lang=\"en\">Assessment of <span id=\"result_box\" lang=\"en\">various compounds, waste<\/span> and products degradation by microorganisms (MT2 plates)<br \/>\n<\/span><\/li>\n<\/ul>\n<p><strong><em>Classical<\/em><\/strong><strong><em> microbiological<\/em><\/strong><strong><em> methods<\/em><\/strong><\/p>\n<ul>\n<li>Total number of bacteria and fungi evaluation in environmental samples<\/li>\n<li>Evaluation number of heat resistant fungi and their isolation from environmental samples<\/li>\n<li>Qualitative and quantitative evaluation of proteolytic, pectinolytic, cellulolytic and amylolytic, microorganisms, ammonifying and nitrifying bacteria in environmental samples<\/li>\n<li>Identification of fungi based on macro- and microscopic observations<\/li>\n<li>Soil respiratory activity evaluation<\/li>\n<li>Assess the sensitivity of fungi to fungicides<\/li>\n<li>Evaluation of the microbiological air contamination using air sampler<\/li>\n<li>Evaluation of water microbial quality using membrane filter method<\/li>\n<\/ul>\n<p><strong><em>Classical biochemical analyses<\/em><\/strong><\/p>\n<ul>\n<li>Enzymatic activity evaluation of bacterial and fungal strains (cellulase, pectinesterase, polygalacturonase, protease, lipase)<\/li>\n<li>Soil enzymatic evaluation using spectrophotometric methods (dehydrogenases, protease, urease, acid and alkaline phosphatase, \u03b2-glucosidase)<\/li>\n<li>Evaluation of ammonification and nitrification rate in soil environment<\/li>\n<li>Evaluation of soil enzymatic activity using fluorimetric methods (leucine aminopeptidase, esterase, acid and alkaline phosphatase, arylosulphatase, \u03b2-glucosidase)<\/li>\n<\/ul>\n<p><strong><em>\u00a0<\/em><\/strong>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text] Preparation and storage of samples Preparation and dispensing of microbiological culture media&#8230;<\/p>\n","protected":false},"author":6,"featured_media":6082,"parent":16209,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"iapan_zaklad_3.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-16352","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16352","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/comments?post=16352"}],"version-history":[{"count":0,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16352\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16209"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media\/6082"}],"wp:attachment":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media?parent=16352"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}