{"id":16477,"date":"2017-03-18T12:30:33","date_gmt":"2017-03-18T11:30:33","guid":{"rendered":"\/en\/departments\/department-of-microstructure-and-mechanics-of-biomaterials\/laboratory-of-biochemistry\/oferta-uslug\/"},"modified":"2017-05-08T11:45:27","modified_gmt":"2017-05-08T09:45:27","slug":"offer","status":"publish","type":"page","link":"https:\/\/www.ipan.lublin.pl\/en\/departments\/department-of-soil-and-plant-system\/laboratory-of-biochemistry\/offer\/","title":{"rendered":"Offer"},"content":{"rendered":"<div class=\"wpb-content-wrapper\" id=\"wpb-content-root\"><p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text]<strong>Sample preparation<\/strong><\/p>\n<ul>\n<li>Isolation of cell walls from different plants<\/li>\n<li>Sequential extraction of polysaccharides from cell walls:\n<ul>\n<li>Pectin fractions: WSP, CSP,ASP<\/li>\n<li>Hemicelluloses fractions<\/li>\n<li>Cellulose<\/li>\n<li>Extraction of natural compounds with ASE 150 with solvents: ethyl alcohol, acetone, water<\/li>\n<li>Sample lyophilisation<\/li>\n<li>Dry sample grinding to the particle size of c.a. 6\u00b5m.<\/li>\n<li>Microbiological medium and small laboratory equipment sterilization<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Traditional chemical analyses<\/strong><\/p>\n<ul>\n<li>Dry mass marking<\/li>\n<li>Acidity<\/li>\n<li>pH<\/li>\n<li>Extract contents<\/li>\n<li>Starch contents, iodine test, enzyme method, polymetric method<\/li>\n<li>Marking the level of pectin estrification with a spectrophotometric method<\/li>\n<li>Enzyme activity &#8211; a spectrophotometric method\n<ul>\n<li>Polygalacturonase PG<\/li>\n<li>Alfa \u2013 L \u2013 arabinofuranosidase (Alfa \u2013 L \u2013Af)<\/li>\n<li>Beta \u2013 galactosidase (Beta \u2013 Gal)<\/li>\n<li>Pectin methylesterase PME<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>Flow analysis<\/strong><\/p>\n<ul>\n<li>Marking the contents of galacturonic acid in water solutions (1\u2013 100 ppm C6H12O6)<\/li>\n<li>Marking the contents of calcium in water solutions (1-100 ppm Ca)<\/li>\n<li>Marking total nitrogen in samples after mineralization (0.1 \u2013 10 ppm N)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>FT Raman and FT IR<\/strong><\/p>\n<ul>\n<li>Identification of organic and inorganic compounds<\/li>\n<li>Defining crystallization level of cellulose in cell walls<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Granulometric analysis of powders<\/strong><\/p>\n<ul>\n<li>50\u00b5m, 100\u00b5m, 500\u00b5m, 1 mm, 2mm, 4mm.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Analytical services &#8211; microbiology<\/strong><\/p>\n<ul>\n<li>Qualitative and quantitative marking of fruit and vegetable microflora,<\/li>\n<li>Gluconacetobacter xylinus bacteria breeding.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>contact: <a href=\"&#x6d;&#x61;&#105;&#108;t&#x6f;&#x3a;&#x6a;&#46;&#99;y&#x62;&#x75;&#x6c;&#115;&#107;a&#x40;&#x69;&#x70;&#97;&#110;&#46;&#x6c;&#x75;&#x62;&#108;in&#x2e;&#x70;&#x6c;\">Justyna Cybulska<\/a>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text]Sample preparation Isolation of cell walls from different plants Sequential extraction of polysaccharides&#8230;<\/p>\n","protected":false},"author":8,"featured_media":6082,"parent":16225,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"iapan_zaklad_3.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-16477","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16477","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/comments?post=16477"}],"version-history":[{"count":0,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16477\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16225"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media\/6082"}],"wp:attachment":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media?parent=16477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}