{"id":16482,"date":"2017-03-18T12:27:40","date_gmt":"2017-03-18T11:27:40","guid":{"rendered":"\/en\/departments\/department-of-microstructure-and-mechanics-of-biomaterials\/laboratory-of-microscopy\/aparatura\/"},"modified":"2017-05-08T11:45:02","modified_gmt":"2017-05-08T09:45:02","slug":"equipment","status":"publish","type":"page","link":"https:\/\/www.ipan.lublin.pl\/en\/departments\/department-of-microstructure-and-mechanics-of-biomaterials\/laboratory-of-microscopy\/equipment\/","title":{"rendered":"Equipment"},"content":{"rendered":"<div class=\"wpb-content-wrapper\" id=\"wpb-content-root\"><p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-14401\" src=\"\/wp-content\/uploads\/2017\/03\/clsm.jpg\" alt=\"\" width=\"300\" height=\"194\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/clsm.jpg 427w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/clsm-300x194.jpg 300w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/clsm-400x260.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/h3>\n<h3>Confocal Laser Scanning Microscope (CLSM) &#8211; FluoView300<\/h3>\n<p>&nbsp;<\/p>\n<ul>\n<li>CLSM allows for micro-structure observation of samples surfaces and 3D imaging. Images are taken only from thin confocal layer, thus images are usually very contrastic.<\/li>\n<li>Main features: Two lasers: Ar 488 nm,\u00a0 HeNe 543 nm, 3 channels (two confocal, one optical), high resolution; XY, XYZ, XYZT scanning modes.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-14402\" src=\"\/wp-content\/uploads\/2017\/03\/Catalyst-1024x681.jpg\" alt=\"\" width=\"300\" height=\"200\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/Catalyst-1024x681.jpg 1024w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/Catalyst-300x200.jpg 300w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/Catalyst-768x511.jpg 768w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/Catalyst-700x466.jpg 700w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/Catalyst.jpg 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/h3>\n<h3>Atomic Force Microscope (AFM) &#8211; BIOSCOPE CATALYST II<\/h3>\n<p>&nbsp;<\/p>\n<ul>\n<li>AFM allows for nano- and micro-structure observation with analysis of mechanical properties at these scales in air or liquid of biological samples.<\/li>\n<li>\u00a0Main features: Contact, tapping, force volume modes; Harmonix mode for mechanical properties imaging; system for perfusion; temperature control, integration of optical and AFM view .<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-14403\" src=\"\/wp-content\/uploads\/2017\/03\/raman.jpg\" alt=\"\" width=\"249\" height=\"156\" \/><\/h3>\n<h3>Fourier-transform infra-red spectroscopy (FTIR)\/Raman spectroscopy system<\/h3>\n<p>&nbsp;<\/p>\n<p>The system includes Raman disperse spectrometer with 532 nm laser, Fourier-transform infra-red Raman spectrometer<br \/>\nwith 1064 nm laser, IR spectrometer and UV-VIS spectrometer.<\/p>\n<ul>\n<li>Structural identification and analysis of chemical compounds, mapping of chemical compound distribution in samples.<\/li>\n<\/ul>\n<p>Examples of use:<\/p>\n<ul>\n<li>Analysis of multi-molecular compounds; analysis of cellulose crystallinity.<\/li>\n<li>Distribution of active substances in pharmaceutical tablets; chemometric polymer analysis.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-14405\" src=\"\/wp-content\/uploads\/2017\/03\/aphelion2_mini.jpg\" alt=\"\" width=\"150\" height=\"120\" \/><\/h3>\n<h3>Aphelion Dev. 4.0 image analysis software<\/h3>\n<p>&nbsp;<\/p>\n<ul>\n<li>Computer analysis of 2D and 3D images.<\/li>\n<li>2D and 3D image transformation, and analyses, including colour, size and orientation of represented objects.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-14406\" src=\"\/wp-content\/uploads\/2017\/03\/microtome_mini.jpg\" alt=\"\" width=\"135\" height=\"180\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/microtome_mini.jpg 135w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/microtome_mini-112x150.jpg 112w\" sizes=\"auto, (max-width: 135px) 100vw, 135px\" \/><\/h3>\n<h3>Microtome &#8211; LEICA RM2155<\/h3>\n<p>&nbsp;<\/p>\n<p>Microtome is a tool for slicing fixed samples with high precision (&lt; 1 micrometer).<\/p>\n<p>Main features: Cutting accuracy &lt;1 micron, fully programmable; possibility to use different blades: glass, stainless steel, sapphire.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-14407\" src=\"\/wp-content\/uploads\/2017\/03\/vibratome_mini.jpg\" alt=\"\" width=\"137\" height=\"183\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/vibratome_mini.jpg 137w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/vibratome_mini-112x150.jpg 112w\" sizes=\"auto, (max-width: 137px) 100vw, 137px\" \/><\/h3>\n<h3>Vibratome &#8211; LEICA VT 1000S<\/h3>\n<p>&nbsp;<\/p>\n<p>Vibratome is useful for slicing soft materials like plant and animal tissues. Surface has decent quality for CLSM or macroscope.<\/p>\n<p>Main features: Adjustable thickness (with 1 micron step), cutting speed and oscillations; batch for sample allowing cutting in buffers; fully programmable; possibility of use simple razors blades.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][vc_separator type=&#8221;normal&#8221; color=&#8221;#e6e6e6&#8243; thickness=&#8221;1&#8243; down=&#8221;30&#8243;][vc_column_text]<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-14408 size-medium\" src=\"\/wp-content\/uploads\/2017\/03\/carrot_MACRO-300x225.jpg?_t=1490023015\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/carrot_MACRO-300x225.jpg 300w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/carrot_MACRO-768x576.jpg 768w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/carrot_MACRO.jpg 1024w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/carrot_MACRO-800x600.jpg 800w, https:\/\/www.ipan.lublin.pl\/wp-content\/uploads\/2017\/03\/carrot_MACRO-700x525.jpg 700w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/h3>\n<h3>Macroscope<\/h3>\n<p>&nbsp;<\/p>\n<ul>\n<li>Macroscope is a microscope using the dark field view. It is designed for observation large sample areas. Sample must be transparent.<\/li>\n<li>Main feature: field of view up to~8x6mm. Useful for evaluation of samples heterogeneity.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][vc_column_text] Confocal Laser Scanning Microscope (CLSM) &#8211; FluoView300 &nbsp; CLSM allows for micro-structure&#8230;<\/p>\n","protected":false},"author":8,"featured_media":6082,"parent":16223,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"iapan_zaklad_5.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-16482","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16482","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/comments?post=16482"}],"version-history":[{"count":0,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16482\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/pages\/16223"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media\/6082"}],"wp:attachment":[{"href":"https:\/\/www.ipan.lublin.pl\/en\/wp-json\/wp\/v2\/media?parent=16482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}